Hi,
I am having an issue with analyzing a next-gen sequencing data set for differential exon usage using DEXSeq. I have two main questions:...
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DEXSeq Partially Unreplicated Dataset: Weird EstimateDispersions behavior
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DESeq estimateDispersions warning messages
I'm using DESeq to compare one condition with 2 biological replicates, with another condition which has only one replicate.
When running...
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DEXSeq error:Failed to set up model frames for genes
Here is how I produced the error:
annotationfile=file.path(inDir,"Homo_sapiens.GRCh37.68.DEXSeq.chr.gff")
esc=read.HTSeqCounts(countfiles=file.path(inDir,paste(rownames(samples),"txt",sep=".")),design=samples,flattenedfile=annotationfile)...