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PJohn started a topic NextSeq reads count

in Illumina (Solexa)

01-19-2016, 10:22 AM
NextSeq reads count

Hi all,

I counted the number of reads in the same sample in 2 different ways:

a) On the sequencer (NextSeq), by counting...

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gauravdube started a topic Non-ATGC characters, small-case characters and lots of 'N's in fastq file's sequence

in Bioinformatics

10-03-2015, 09:11 AM
Non-ATGC characters, small-case characters and lots of 'N's in fastq file's sequence

Hi All,

My fastq file consists of lot non-ATGC characters. What are these characters and how to handle these?

Commands...

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