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biodiverse started a topic Discrepancies in Demultiplexing programs - help request

in Bioinformatics

09-18-2017, 07:38 AM
Discrepancies in Demultiplexing programs - help request

I have Illumina PE data from a MiSeq run and am finding large discrepancies in the number of reads recovered while demultiplexing.
Originally,...

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biodiverse started a topic Large Discrepancies in Different De-multiplexing programs - help request

in Bioinformatics

09-18-2017, 07:15 AM
Large Discrepancies in Different De-multiplexing programs - help request

Hello,

I am working with Illumina PE reads from a MiSeq run and am having trouble correctly de-multiplexing them.
The fastq files...

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a_mt started a topic effective mismatches allowed while demultiplexing

in Bioinformatics

07-11-2013, 08:16 PM
effective mismatches allowed while demultiplexing

Hi all,

What is the minimum/optimum number of mismatches to be allowed during demultiplexing ?? How do we decide this?

...

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jdanderson started a topic FASTX Toolkit barcode splitter issue

in Bioinformatics

10-03-2010, 06:18 PM
FASTX Toolkit barcode splitter issue

Hello All,

I've been trying to use the FASTX Toolkit barcode splitter to demultiplex my illumina reads. The following command runs okay...

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