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FLX+ Library Amplification Issue
We are having problems getting our long-fragment shotgun library to amplify during emPCR. Titration using 3 different cpb ratios (12, 18, and 24) yields...
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The new flow pattern for FLX+
Has anyone updated their FLX+ machine to 2.8 software with the new flow pattern? Does it work? We are very reluctant, mainly because last time roche touched...