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nupurgupta started a topic SAM flag forward/reverse first/second

in Bioinformatics

04-27-2012, 07:23 PM
SAM flag forward/reverse first/second

Hello,
I am a little confused with the interpretation of SAM flags
From http://ppotato.wordpress.com/2010/08...wise-flag-pair...

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ct586 started a topic rmdup can not move duplicates in forward and reverse strand for single-end reads

in Bioinformatics

03-11-2012, 06:35 AM
rmdup can not move duplicates in forward and reverse strand for single-end reads

Hi, I have a sam file produced by BWA for single-end reads.

There is one type of results like the following:
...

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Last edited by ct586; 03-11-2012, 06:41 AM. Reason: To make the words more accurately
darthsequencer started a topic Forward adapter at end of forward paired end read?

in Illumina (Solexa)

03-01-2012, 08:46 PM
Forward adapter at end of forward paired end read?

So I was given a metatranscriptome prepped with the Nugen Ovation RNAseq kit.

The metatranscriptome was sequenced on a HiSeq 2000 paired...

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