Hi,
I used hisat2 (2.0.5) to map 150b Paired-end reads on to the draft genome of castorbean and used cufflinks (version 2.2.1) to perform reference...
2 results in 0.0020 seconds.
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with cufflinks, can there be "0" fpkm value and yet full read support?
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FPKM=0 in cufflinks - but there are reads mapped to these genes!
Hello everyone
We are using BAM to map Illumina reads to a bacterial genome, followed by Cufflinks to get the FPKMs (implemented in Galaxy)....