Hi all,
I've been working for some months to collect samples and build libraries for an absolute RNA-seq method, which is preferably PCR-free....
8 results in 0.0030 seconds.
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Options for poorly fragmented library.
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Estimating the number of ddRAD fragments
Hi all,
I am wondering if there is an easy way to estimate the number of fragments resulting from a double digest reaction (in my case... -
TristanB started a topic Compare avg fragment length of major/minor allele reads at heterozygous positionin GeneralCompare avg fragment length of major/minor allele reads at heterozygous position
Hi all,
I would like to compare average fragment length among reads contributing to a minor allele at a given heterozygous position vs... -
generating pileup of paired end fragments
Dear all,
i'm working with paired end rnaseq data. i wanted to generate a pileup with samtools mpileup but noticed that only the mapping reads get...Last edited by Slacanch; 01-20-2015, 08:42 AM. -
Automated vs. Manual fragment library preparation - need script!
Hi,
We bought "48 fragment library construction kit" for Solid 5500, which is supposed to be used in automated systems like Tecan, but... -
Fpkm
I was wondering what does FPKM means in REAL English? "fragments per kilobase of exon per million fragments mapped" clearly means nothing to... -
latest SAM format: did the meanings of read and fragment get swapped?
In the latest SAM format specification, the meanings of "read" and "fragment" seem to be reversed from what I am accustomed to.... -
IndelRealigner on fragment data
Hi everyone,
I want to locally realign some bam files coming from exome fragment data with the GATK IndelRealigner tool. I have done the...
