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Can Cufflinks report chimeric transcripts/fusions?
I understand that Cufflinks can report FPKM on novel transcripts (different isoforms) if using proper parameters, but I wonder whether cufflinks is able...
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ViReMa: an algorithm for finding viral recombination or fusion events in NGS data
Hi all,
I have written and made available an algorithm I wrote in Python called ViReMa: a Viral Recombination Mapper.
The...
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Secondary Alignments with BWA-MEM
When I have split reads originating from repetitive sequences I must use the -a flag to get the secondary alignments. The problem with this is that with...
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Cufflinks non-model organism, issues with -b, and false concatenation of genes
Hello,
I have been working with the "tuxedo" package for a little while now, and have started getting the nice results that I want,...