Hi guys,
We are using NGS to detect non-clonal, low frequency mutations, so we are quite concerned about the background the sequencer introduces,...
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Illumina GAIIx sequecing error rate (background)
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Used/decomissioned GAII/GAIIx sequencers wanted
Has anyone out there decomissioned a GAII/GAIIx and want to get rid of it (cheaply). I have a use for some of the parts inside and would happily pay for... -
Maq error after using cutadapt
I have some small RNA data, generated with a GAIIx, and I need to trim the adapter sequence off the 3' end. I used cutadapt, which seemed easy enough,... -
CASAVA v1.8 with indels
My Settings :
Biology : GAIIx, SureSelect capture, paired-end 2x76 with "in house" indexing
BioInformatics : CASAVA... -
Sequencing ChIPseq samples prepped with micrococcal nuclease?
I was wondering if anyone has had any issues sequencing samples that were prepped using a micrococcal nuclease?
I ran a flow cell that... -
how MUCH data do you give back?
Hi all!
We're a relatively new core running Illumina's GAIIx and I'm trying to deal with the issue of data. Typically once the customers... -
Actual adapter sequences of the HiSeq2000 flowcell?
Hi,
I am looking around to find the actual sequence of the adapters present on the flowcell of the HiSeq2000. Does anyone know this?... -
Illumina GAIIx available
I have an ILLUMINA GAIIX 2009 available selling to upgrade for purposes of upgrading to a HISEQ. Contact me if interested. -
RTA hangs in the middle of GAIIX run
We started a PE run but to my dismay RTA stopped in the middle of the run. After the first read, we stop the run and restarted RTA but it did not help.... -
GA IIx - Focus was not found
We're performing our first run on an Illumina GAIIx (after "training" run) and GA Data Collection software can't focus showing the following...
