Dear all,
We are pleased to announce our upcoming online course “Generalised Linear Models in R”, taking place 25–29 May.
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Generalised Linear Models in R course
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RNA-Seq Time series analysis in EdgeR
Hello,
I have an RNA-Seq experiment from insect antennae across different data points (0,2,4,6 and 8 days) with 7 replicates per data point... -
Choosing experimental models for three-condition DE analysis
I recently got a new project to work on regarding differential expression analysis of RNA-seq data, and it's slightly different to what I've done previously... -
Comparing multiple treatment groups for DNA methylation (RRBS, methyl kit, GLM)
We have an RRBS dataset with 4 treatment groups, n=6 samples each group. I'd like to be able to do an ANOVA-style first stage analysis to look for evidence... -
DESeq2 and GLM with grouped samples in two conditions
Hi all,
I came across this post from Simon Anders, suggesting to use a GLM if one has grouped samples across the conditions.
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RNAseq normalize DE by edgeR with transfection positive control gene
I am trying to normalize my RNAseq data to take into consideration transfection efficiency. I have two conditions that were applied to cells in culture,... -
model matrix design for multiple siRNAs targeting same gene
Hi,
after lurking here for 2 years or so (and learning so much from this community!) I finally have a question I haven't been able to find an... -
Help with multi factorial model fitting in edgeR/DEseq
I have two different groups which has been followed over a time course. During this time six samplings has been done. I then have two levels of groups... -
EdgeR GLM linear regression of time series
Hi All,
I am using RNA-seq data. I have 3 time points in my data 6 months (young), 12 months (middle) and 28 months (old). I want to do... -
GLM in DESeq
Hello;
I am using the nbinomFitGLM and nbinomGLMTest in DeSeq, which have worked well. However, I would like to know if both of the factors...
