Hi guys,
a quick question. I am new to pacbio. I've just received pacbio data for assembling a genome (low coverage). It comes from a highly...
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Pacbio assembly of polymorphic genome
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Heterozygous rate from "de novo" sequencing
Hi,
I've just tried to calculate the heterozygous rate of my seq data, but no luck using gce or even preqc from sga using kmer information.... -
classifying a variant as het or hom
Hi all,
I have a general question. Assume I have a genomic position which had nice sequence coverage and quality at the position (i.e. all metrics... -
gffread - heterozygotes and indels
Hi all,
I am trying to get .fasta files for each CDS in a .gtf file and the most obvious way I can find of doing it is to use gffread and... -
detect DNA damage in NGS data
Hi ,
We have conducted an RNASeq experiment (control vs bacterial infection 3 replicates : host) and identified a few DE genes in the host... -
MAQ: SNPs interpretation, etc!
I'm a graduate student just starting to work with Maq for calling SNPs from Illumina resequencing of the Giardia genome. I'm a bit unsure about appropriate...
