Hi,
I'm very new to sequencing so apologies if my question is naive.
I am trying to sequence whole human genome samples...
65 results in 0.0032 seconds.
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Single index adapters for low-plex
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third party illumina equipment
Has anyone else had trouble getting Illumina to service or support instruments not bought directly from Illumina? Any feedback on software license transfer?... -
Refurbished Hiseq 4000, Nextseq 500, Hiseq 2500v4 & Miseq DNA Sequencers <Sponsored>
Greetings,
We have the following systems in-stock. Please message me if you are interested.
Like-new Illumina HiSeq 40... -
Trimmomatic: Not trimming..
Hey! I am trying to use Trimmomatic to trim Illumina adapters sequences from my TruSeq Small RNA library SE 75bp reads off of a HiSeq but I am unsuccessful......Last edited by lchippy; 11-12-2017, 11:34 AM. -
Custom primers for NextSeq: will they work on MiSeq and HiSeq?
I am wondering whether my experiment (which was originally designed to work on a NextSeq) will work on MiSeq and HiSeq.
It is a library... -
Custom primers for NextSeq: will they work on MiSeq and HiSeq?
I am wondering whether my experiment (which was originally designed to work on a NextSeq) will work on MiSeq and HiSeq.
It is a library... -
NGS service Boston area
Hi Fellows NGS users,
I want to introduce myself. I am Peter. I have been in NGS development for quite some time. Started HERA Biosciences...Last edited by ianakiev; 07-19-2017, 10:24 AM. -
PCoA Plots
Hi Everyone,
I have HiSequencing data that I want to set up PCoA plots for.
These are environmental samples and I want to look at the diversity... -
Number of Reads
Hi there,
I have Illumina HiSeq fastq output files. I want to know how I can find out the number of reads per sample before and after processing... -
pipeline optimization for NextSeq vs Hi/Miseq
Dear all,
I heard comments lately, from two sources, that NextSeq 500 "requires extensive bioinformatics pipeline optimization and... -
FastQC Report
I ran a HiSeq on environmental samples and the purpose of the run was to blast my sequences against the NCBI-nr database to see what species my reads... -
TruSight One - Hiseq Loading Concentration
Hello,
I had a quick look through the pre-existing threads but couldn't find an answer so forgive me if it's already been answered! ... -
How much phiX to spike in for ddRADseq library on HiSeq?
Our lab is preparing for a large scale ddRADseq project (~3000 samples). In doing the calculations for how many lanes of sequencing we will need to achieve... -
Three reads with the same name in the BAM file
Hi all,
I am dealing with the paired-end BAM file, and come up with many warnings like this:
...Code:WARNING: Could not find pair
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illumina aligment ratio reduced when increase insert size
We build two different cDNA library for the same sample with different size(196 and 225) for pair end sequencing by Hiseq with the same sequence length...
