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How many lanes do I need?
I will be performing an RNA-seq experiment for differential gene expression with Illumina HiSeq 2500 (100 or 150bp pair-end). My experiment is designed... -
Does cell type affect possibility for multiplets during GEM formation?
Hi. Do the cell types to be sequenced by single cell RNA sequencing have a possibility to influence the occurance of doublets during the creation of GEMS... -
Is the HiSeq2500 DOA?
Is the HiSeq2500 being phased out? In other words, has it reached EOL and/or is Illumina ceasing to provide supply reagents?
Thanks,... -
Refurbished Hiseq 4000 & Hiseq 2500v4 in-stock
Greetings,
We have the following two systems in-stock. Please message me if you are interested.
Like-new Illumina HiSeq... -
Processing a very large soil dataset
I’ve been tasked with assembling a very large soil metagenomic dataset, so large that I’m having second thoughts about my regular processing pipeline,... -
polyA sequencing
Hi, I hope to do a polyA enriched RNA-seq on Hiseq2500, which only sequence 3' tags with polyA tails. I realize the polyA tail will affect the sequence... -
Adaptor Removal with Trimmomatic
Hi there,
I ran a HiSeq2500 run and found that I had adaptor sequences still present.
I first used Trimmomatic to simulatenously... -
TruSight One - Hiseq Loading Concentration
Hello,
I had a quick look through the pre-existing threads but couldn't find an answer so forgive me if it's already been answered! ... -
sequencing paired-end and mate-pair libraries in a single lane?
Hi folks,
We are new to genome sequencing, but we want to sequence fungal genomes using Hiseq. We were wondering if it is possible to... -
HiSeq4000 small RNA libraries (vs 2500)
Hi All,
I'm having some issues running a custom small RNA sequencing protocol on the HiSeq4000, when the same library works perfectly fine... -
HiSeq rapid run overclustering
Hello,
On the Illumina website, it says that the optical raw cluster density for PhiX runs is 850K-1000K clusters/mm2. But, what is the functional... -
Microbial Metagenomics: Nextera. Nextera XT, or TrueSeq to run on HiSeq 2500?
We have 96 gut microbiome samples that we want to run using the Nextera XT or Nextera Kit. Most of our samples have between 1 - 40 ng of DNA. Would it... -
Failed second index. Any chances to get some data?
Dear colleagues!
We have conducted a HiSeq 2500 dual index paired end run using TruSeq SBS Kit v3 - HS (200-cycles) chemistry and TruSeq PE Cluster... -
HiSeq 2500 v4 chemistry Read 1 quality drop
We have been seeing a very specific fail mode in about 1 in 10 runs since upgrading to v4 chemistry last year, and we wonder if anyone else is experiencing... -
HiSeq2500 - how to sequence pair after single end run
Our machine was prepped for a Paired End run, but we forgot to enter the read length for the paired read, so the machine ran it through as single end....Last edited by dlawrence; 02-18-2015, 06:06 PM.
