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NextSeq having problem sequencing amplicons with stretch of C
We seems to have problem sequencing several different amplicons with a stretch of C on the NextSeq using v2 chemistry 300-cycle kits. This happen to 2... -
homopolymer, steps after alignment
Hello.
I am implementing my pipeline and I have to start the steps after alignment. I have see on GATK pipeline that I have to do local realignment... -
lowercase output from quiver
I recently completed resequencing of a bacterial strain with a complete reference genome. The HGAP3 assembly resulted in a single contig, but there were... -
454 Pyrosequencing error rate assesment tool
Hi,
Is anyone aware of some software or documented algorithm for estimating (separately) the error rate of PCR and sequencing for 454 data,... -
New Software for Homopolymer Identification
Hello,
We are in the process of releasing a software tool SCOPE++ (Sequence Classification Of homoPolymer Emissions).
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Pileup - Filter SNP/Indel linked to 454 homopolymer
Hi all,
With 454/Roche the most common mistake is insertion/deletion errors in a homopolymer.
Do you know "tool" to filter...
