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Confusing Picard HsMetrics ON_BAIT_VS_SELECTED for near=250 and near=0
Hi, for an assay developed by my vendor, I ran latest Picard HsMetrics (2.21.8) with two different options for the same set of BAM files: one with a NEAR_DISTANCE=250...
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Dictionary size problem in Picard HsMetrics
Hi everyone,
I'm following a Roche pipeline for the analysis of targeted bisulfite sequencing data and I'm in trouble with Picard CalculateHsMetrics....
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HS_LIBRARY_SIZE vs. ESTIMATED_LIBRARY_SIZE
Nothing came up on a search here today for me, so I dug into these Picard modules.
Looks like MarkDuplicates.ESTIMATED_LIBRARY_SIZE estimates...