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Method for Identifying Adapters??
I have been given Illumina paired end sequences for a chicken genome. I do not have access to the experiment protocol and the adapter files nor do I...Last edited by nemis00; 10-17-2015, 05:32 PM. -
inline barcodes appearing in reverse read of pair
Hi All,
I have Illumina 101bp paired-end data where the libraries were prepared with custom inline barcodes at the P5 end. So the first... -
Is end repair and 5’phosphate necessary for the adaptor ligation?
Hi,everyone! I’m new here and this forum has helped me a lot,thanks.
We are now hoping to carry out NGS of PCR amplified DNA fragment. The DNA... -
Fastq quality line symbols
Hello everybody. I got a (stupid?) question: when you open a fastq file and you look at the quality line, how do you know which symbol represents a good...
