21 results in 0.0036 seconds.
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Miseq
Hello, I work with the Miseq from illumina, I have been looking all around for an answer but cannot find one. When you open the reagent cooler door is... -
% PhiX for Illumina sequencing protocols and SAV interpretation?
Hello,
I am a bit confused on how to calculate the volume of PhiX to add to the sample prior to sequencing on the Illumina platform. Based... -
Cutadapt Demultiplex
Hi all,
I'm working in R and trying to demultiplex some data using cutadapt
I have three files:barcodes.fastq.gz (which I have... -
MiSeq available being retired
770-490-3265 we have one that is being retired. A illumina MiSeq Excellent condition. -
Basequality Dropoff
Hello people,
first time poster and also kind of first time sequencer.
I just started my PhD which involves a lot of NGS on a MiSeq... -
Where to start for sequence analysis of 24 virus Illumina Miseq
Hello Folks
We have run 24 influenza virus samples on our MiSeq and obtained 48 FASTQ files (R1 + R2 for each). We also got 24 Contig files.... -
Short mixed amplicon libraries
I'm interesting in sequencing libraries composed of multiplexed PCR products with amplicon lengths running from 50 - 150 bp. A colleague expressed concern... -
Alignment of reads obtained from HaloPlex HS target-enriched sequencing
Hello everyone,
I have reads that are obtained from HaloPlex HS target-enriched sequencing (Illumina MiSeq platform).
As per my understanding,...Last edited by vandana8; 06-20-2017, 10:54 PM. -
Failed index reads with homegrown adapters
Hi,
I'm hoping someone can help. I am trying to sequence amplicons on the MiSeq with adapters and barcodes we have designed in the lab.... -
multiple occuring element cause problem with scaffolding
Hello,
I have problem with scaffolding. I tried to combine Spades and SSpace but after several failures a gave it up. I realized that the... -
MiSeq Pooling\multiplexing 240 samples using Nextera v2 Index
Hi everyone,
I am following Illumina's 16S protocol for sequencing and I use Nextera XT Index v2 adapters kit. I do my runs on MiSeq using... -
Hello everyone!
Hi!
I am Aleksandra and I work on microbiome of ticks. Since the last year I am using NextGen (Illumina MiSeq) to produce huge amounts... -
Best/ cheapest Platform for RNA-Seq
I am a MS student working with soil microbiome. I am looking for the presence of a gene family in soils under certain conditions and would like to use... -
Quality trimming & filtering illumina reads
Hi,
I have a illumina MiSeq data set, 32GB size genome, 300bp reads. Quality of reads degrades towards the 3' end in both R1 & R2,... -
homozygous variants on X and Y choromosome of male patient
Hello,
I am relatively new to the field of NGS so I will appreciate any help with data analysis.
I have a vcf file sent...
