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Trimming stringency w/ excessive data
I'm in the process of assembling a 500Mb animal genome and have both Illumina and PacBio data to work with using a hybrid assembly approach. Generally...Last edited by adamrork; 12-12-2019, 08:49 PM. -
Picard IlluminaBasecallsToSam: Read Structure
Hi,
I am trying to convert MiSeq .bcl files into uSAM with Picard tools 'CheckIlluminaDirectory', 'ExtractIlluminaBarcodes' and 'IlluminaBasecallsToSam'.... -
Creating psuedo paired-end sequencing reads from single-end reads
Does anyone know of a program out there that will generate psuedo paired-end sequencing reads from Illumina 100bp single-end reads? This is for an initial... -
How short an Illumina read needs to be discard?
I'm working on ancient DNA mitochondrial genome mapping. We extracted genomic DNA from "fossil" bones. Then we did paired-end Illumina Hiseq... -
How are mate pairs formed and stored
Lets say I have a genome G=ACTTAGGCTA. So mate pairs as far as I know are two reads in the genome which are separated by a known distance. Consider read... -
Challenging read mapping problem
Hi,
I have about 70Gbp of paired-end data from potato [1] that I'd like to map to one of the chromosomes (chr04). I only want pairs where...Last edited by dan; 09-18-2012, 09:09 AM. Reason: Either end or both ends can match, and I want to return the pair. -
Bisulfite analysis with Illumina FastQ from different lanes
Hi everybody,
I'm a newbie in NGS bisulfite Methylation analysis, so first, apologies iof the question is so simple but I couldn't find... -
How to fix Illumina FASTQ files with read length varies errors
How to fix FASTQ files with read length varies errors
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Dear List,
I am a newbie in NGS analysis, I've got... -
Why Illumina reads may have uneven coverage?
Why Illumina reads may have uneven coverage of the genome? Does it depend on GC content of the genome, and if so, then why and how exactly?
I...
