Hello everyone!
We designed Unique Dual Indices, with both nextera adapter sequences and truseq so we can see which performs better, in the...
65 results in 0.0036 seconds.
-
My Unique Dual Indices with Nextera adapters, dont bind with any library preparation method
-
Help in understanding the Demultiplexing summary files
Hi all,
I'm working on analysing my RNA-seq data from NextSeq 550 machine.
Among all the things I'm trying to understand, there... -
Use Illumina UDI's in a combinatorial fashion?
Hello All,
I am wondering if someone has used selected UDI sequences such as the IDT UDI 10 nt long barcodes in a combinatorial fashion and...Last edited by michael10275; 03-03-2021, 07:27 AM. -
i5/7 (P5/7) Nomeclature
Why is i5/7 (P5/7) named 5 and 7?
I understand i5/P5 are on 5' end, so perhaps it makes sense to name it 5, but what about 7? Or it must be some... -
Split seq data based on index AND barcode
Dear all,
I am currently designing an experiment, where my samples will have both a barcode and an index.
Barcode: Sample-specific...Last edited by Meyana; 01-26-2020, 08:25 PM. -
10x genomics 3'RNA-seq dual indexing
Hello,
so I had a discussion with the 10x Genomics support people about sequencing the v3 chemistry 3'RNAseq libraries on a HiSeq3000/4000... -
Bowtie-build - reference genome issue
Hello!
I`m new in NGS analysis, but in my new lab i had to replace my colleague in big NGS project related to miRNA-seq. He left me bunch... -
MapSplice2 does not recognize my Bowtie indexes
Hi everybody,
I wannted to start working with MapSplice2, however, I run into the problem that it does not recognize my Bowtie1 indexes... -
How to regenerate an index file for a gzipped vcf file?
I have a large vcf file with genomic data (330 GB) and an index file that accompanies it. I ran the command:
bcftools view -f PASS... -
MiSeq-no reads mapped to index ID
Hello there,
recently I performed a MiSeq run (v3 kit, standard flow cell) with 40 samples and faced some difficulties. First of all, we... -
Tandem dual index- low Q30 for index 2
Hello,
While reading up about index hopping, I came across the Illumina whitepaper (https://www.illumina.com/content/dam/illumina-mark... -
Multiple indexes per sample how to trim with Trimmomatic?!
Hi all,
I received my HiSeq Illumina RNAseq data and want to run trimmomatic to remove bad quality reads and trim adapters. I have used... -
Minion sequencing
Hi all,
I have a very basic question. We have ±10 samples that we would like to sequence using MinION. These are PCR amplicons that we... -
best way to index tab-delimited text file
What is the best way to index a tab-delimited text file containing chromosome, position and variant data? My files are huge and too big to maintain in... -
Hisat2 index with splice sites
Hello,
I would like to detect lncRNAs from RNA-seq data. Initially, I'm using Hisat2 for aligning the samples. For this I downloaded the...
