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How to create a gff3 file with introns for MISO
I've got RNA-seq data (Homo sapiens, single ended, 75 bp, Illumina) and want to look for general missplicing - intron retention (IR) - in a given condition...
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Isoform tracking in Cufflinks
Hi all,
I am using Cufflinks to look at differential expression of alternative splicing. The big problem that I'm running into is that...