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jlayer · 03-13-2014, 10:30 AM

Hello All,

I'm a 2nd year graduate student studying DNA repair. I want to sequence an amplicon library of translocation junctions to quantify...

Julien Roux · 03-26-2013, 07:32 AM

Dear all,

I have a question regarding junctions discovery in Tophat (version 2.0.6). I have mapped an unstranded RNA-seq library (single-end...

SequencingWhore69 · 03-24-2013, 08:46 PM

Hi everyone,

I am using paired end 100 bp reads on an illumina hiseq platform to find chromosomal rearrangements. I can find them just fine...

splaisan · 06-27-2011, 07:29 AM

(modified from the BioStar initial post)http://seqanswers.com/forums/images/smilies/smile.gif

I am looking for code/tools to merge and...

Dario1984 · 06-15-2011, 07:00 PM

Hello,

I was getting some strange results with cufflinks, where exons were being called as separate transcripts, so I delved deeper, and...

altodor · 01-12-2011, 07:26 AM

Hi all,

I ran TopHat, it aligned reads but couldn't find any junctions! So I received files with left-hand reads and right-hand reads...

zun · 10-21-2010, 06:55 PM

Hi, everyone

I am a little confused about bowtie index files.

I am treating Arabidopsis and Oryza RNA-seq data....

jm1234567890 · 04-15-2010, 01:37 PM

Hello SEQanswers Community,

Further Info: http://www-stat.stanford.edu/~kinfai/SpliceMap/

We at the Wong Lab have developed...

jling · 02-10-2010, 11:26 AM

I tried using Tophat for paired end analysis.

[Tue Feb 9 23:26:32 2010] Mapping reads against m_musculus with Bowtie
[Tue Feb ...

Sequencing Translocation Junctions