Has anyone else experienced this base composition bias during ligation? Check out figures 1 & 2:
Ligation Bias in Illumina...
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Ligation Biases during library construction
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Optimized Directional RNA-Seq Kits Incorporate Bias Reducing Technology
The patent pending NEXTflex™ Directional RNA-Seq Kits and Barcodes Primer Sets provide an easy, flexible and non-biased solution for generating directional...