Hi all,
I have been getting some questionable runs from a sequencing facility and was wondering if there could be any clues as to whether...
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Low data output from questionable sequencing facility
Last edited by samd; 11-06-2019, 06:46 PM. -
Hybrid assembly with low coverage PacBio libraries
Hi everyone,
We are working on sequencing the genomes of a few bacteria and microalgae. We recently had a PacBio run which provided pretty... -
reggiardo.anselmo started a topic Low Coverage using target enrichment on Ion Torrentin Ion TorrentLow Coverage using target enrichment on Ion Torrent
Hi everybody.
Hope you can help troubleshooting this issue.
I used to carry our BRCA1/BRCA2 target enrichment using Ampliseq paneles. However,... -
Tool for viral metagenome assembly with extremely low coverage?
Hello!
I am studying the interaction of bacteria with plasmids and bacteriophages. I have faced with the following problem. I have 30-bp reads... -
Very low abundant genes, interpretation of noise
In a typical RNA-seq experiment, Illumina paired-end, Hs ensembl genome, happy tuxedo mapping and HTSeq counting, we get a list of genes associated with... -
Trying to identify low coverage regions (bases) in my sample from a Bam and BED file.
I have a bed of my targets (start and stop coordinates of each exon of my genes of interest). I have a BAM file generated from my ION PGM run. I am... -
Dealing with overlapping read pairs
Some of our whole-genome libraries end up with low insert sizes (e.g. ~150) for 2x100 bp sequencing with Illumina HiSeq. I'm concerned about the effect... -
How to find sequence in fragmented assembly
best wishes from a Newbie,
please any advice from someone working on large plant genomes:
What is the standard , best approach...
