Hi,
We would like to do differential expression analysis, we did a genome (Illumina + PacBIo) and a transcriptome (Kapa mRNA kit) but GC-rich genes...
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Ransom Library prep for GC-rich genes
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Is it possible to let Cufflinks avoid discarding low expression transcripts?
As we not, Cufflinks will automatically discard some transcripts with low expression. I wonder if it is possible to avoid doing this? Thanks.