Dear community,
I am currently analysing a set of stranded paired-end RNA-seq data (human, 80 bp). Up till now, I only trimmed the adapter...
4 results in 0.0028 seconds.
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High percentage of STAR unmapped reads: too short
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Selecting the best alignment BAM file
Hi,
I have a PE dataset 300bp inserts by illumina MiSeq. I aligned the raw data using BWA-mem. Mapping statistics generated using Samtools... -
How can I determine the mapping rates of tophat output such as accepted_hits.bam?
I used TopHat to run the same RNA-Seq data with different -r/--mate-inner-dist and --mate-std-dev.
Here are the parameters:
1.... -
Mapping statistics from fragmented FFPE samples using Ribo-Zero
Recently we have performed RNA sequencing using Ribo-Zero in order to remove rRNA from fragmented FFPE samples. The number of reads passing filter are...
