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znasim09 started a topic Can the "QNAME" information of a SAM file be used for estimate uniquely mapped reads?

in RNA Sequencing

08-02-2018, 07:07 PM
Can the "QNAME" information of a SAM file be used for estimate uniquely mapped reads?

Hello everyone,

First of all, I know there has been a lot of discussions related to uniquely mapped reads estimation (sorry for bringing...

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Last edited by znasim09; 08-02-2018, 07:09 PM.
Rangika started a topic Low coverage after alignment

in Bioinformatics

09-28-2016, 07:57 PM
Low coverage after alignment

Hi,

I'm working with a illumina Miseq 2*300bp PE 8 sample library run on a single lane of a flowcell. Ref ~32Mbp in size. I aligned PE...

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ruchi1621 started a topic Getting mapping statistics from a .bed file

in Bioinformatics

10-24-2013, 04:24 PM
Getting mapping statistics from a .bed file

Hello,
I have a .bed file that has the aligned sequence reads for some regions of interest. I want to find the number of reads aligned within the...

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