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Optimal coverage for sequencing microsatellites with Illumina
My lab is planning a phylogeography study on several different groups of lizards using microsatellites. We are interested in pooling the microsatellite... -
Developing microsatellites from pacbio reads
Disclaimer: everything I know about Pacbio has been learned over the process of the last few days. I'm basically the closest thing to a bioinformatician... -
Primer3 primer3_core failed
Hi, I select 11 microsatellites generated by MISA program (http://pgrc.ipk-gatersleben.de/misa/), now I want to design primer, and I use this.
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kpcoats started a topic What program do you use/recommend to mine microsatellites from Ion Torrent data?in Ion TorrentWhat program do you use/recommend to mine microsatellites from Ion Torrent data?
What program do you recommend to mine microsatellites from Ion Torrent data? -
QDD v3.1 users: Pipe1 settings?
I'm curious if any of you are using QDD v3.1 to mine for microsatellites, specifically from Ion Torrent assembled data? We have a pile of contigs assembled... -
Single Nucleotide Repeat Help Needed
I am trying to find the "true" genotype of several loci from target capture data. These are single nucleotide repeats between 15-18 bps long.... -
strains identification by STR micrsatellite using NGS
We have many vials that contain mixture of yeast STRAINS (of 3 or more strains). Currently we are using Capillaries Electrophoresis (CE). With culturing... -
PCR amplifying microsatellites (short tandem repeats) for sequencing
Hi all,
I'm looking to amplify specific dinucleotide repeats from low input DNA (~1ng) but I'm having problems with microsatellite contraction/expansion... -
Fragment analysis; microsatellite or SSR/STR
Has anyone been using next gen or preferably illumina for fragment analysis.
Right now my project are running multiplex for Sanger 3130xl machine... -
microsatellite query
Hello wise people!
I have approx 10,000 microsatellites that I obtained from some assembled dna of a non-model organism. I selected a subset... -
sequencing microsatellites
Dear all,
I was wondering if any of you have information on sequencing microsatellite repeats (amplicons) on a 454 platform.
...Last edited by koen de gelas; 07-27-2011, 11:43 PM.
