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Library with strong secondary structures
What is known about the strand displacement activity of the DNA polymerase Illumina uses for bridge amplification? I have a library with strong secondary... -
non-equimolar pooling to normalise read depth in libraries with different genome size
I'd like to sequence libraries of very different genome size on a single MiSeq run.
Eight bacterial samples with 4.5Mb genome size
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index diversity on MiSeq
Can I run 2-3 index combinations on MiSeq? I'd be using Nextera XT DNA sample preparation index kit and MiSeq v2 reagent kit. If I select index pairs...
