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MISO annotations
I have downloaded MISO annotation files for hg19, applied the MISo algorithm for a BAM aligned to hg19, and noticed that some annotations' event name...Last edited by rlv; 02-02-2017, 12:20 PM.
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MISO: Best-guessing insert size and stddev versus running single-ended for PE data
I am conducting a miso analysis for a single gene on a large number of Paired End RNAseq bams that is spread out on several external HDs (twenty 4tb drives)....
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Differentially expressed isoforms with Miso
Hi all,
I was recommended Miso for alternative splicing analysis. Now, I have a question on how to do this in comparison with two groups...Last edited by ninni; 01-12-2016, 01:29 AM.
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How to get genome wide statistics with MISO
I've done work with specific genes using MISO and obtaining various isoform/exon-centric outputs and visualizing with sashimi plot. All that has been...
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Problem generating gff3 exon centric annotation to run MISO
I would like to use MISO to extract differential alternative splicing in my rat RNA-SEQ samples, but unfortunately there are not gff3 files for Rat. Has...
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MISO output - mismatch between number of transcripts and number of assigned counts
I'm using MISO (v 0.4.9) to look at changes in isoform usage in some mouse RNA-seq data. I need to parse out the number of reads assigned to each isoform...Last edited by MikeStubbington; 01-29-2014, 08:42 AM.
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Miso/Sashimi python error
Hello! New to forum and bioinformatics...
I've run RNA-seq quite deep (100-PE, ~200 M reads/samples) on primary tumors, looking at splice...
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MISO comparing two conditions for DEU
Hi All,
I am considering using MISO for testing differential exon usage (DEU). I have two conditions, control group and treatment group,...
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splicing variant methods and their pros and cons
Hi All,
I read a post here about isoform expression quanification from RNA-Seq, and was really impressed by the summaries. I am working...
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How to create a gff3 file with introns for MISO
I've got RNA-seq data (Homo sapiens, single ended, 75 bp, Illumina) and want to look for general missplicing - intron retention (IR) - in a given condition...
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Anyone Tried MISO with Posted GFF files?
I am trying out the Burge lab's MISO program for isoform analysis. I want to use the mm9 file posted on their website, but within the mm9 folder are...