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Multiplex PCR missing some loci/low template
I am trying to multiplex 12 primer sets in a single PCR. The goal is to amplify 12 taxon-specific loci from environmental samples. So, 1) DNA concentrations... -
Multiplexing PCR for targeted sequencing
Dear All,
Has anyone done multiplex PCR for targeted sequencing on MiSeq?
What software have you used to design the primers?...
