Hi All,
I'm trying to get an idea of the processivity of Nanopore. For instance, do the pores reliably read the full length of 5 kb DNA...
47 results in 0.0028 seconds.
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Nanopore: Input cDNA Length vs. Read Length?
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Nextflow workflow for ONT reads QC, Assembly, Polishing and Evaluation
Can anyone suggest if there is any comprehensive snakemake or Nextflow workflow for ONT reads QC, Assembly, Polishing and Evaluation? -
errors at the beginning of hybrid assembly using MaSuRCA-4.0.3
Hi
im trying to make hybrid assembly for Ecoli using MiSeq and Nanopore data in Masurca. currently i m getting two errors marked in Bold... -
Nanopore direct RNA Sequencing course
Dear all,
last seats available on our course on "Introduction to RNA Sequencing", which will held online from the 18th to the... -
Nanopore library on top of an Illumina library
Hi all,
we don't have the original aliquote of an old sample, but we have an Illumina Nextera Flex library in the freezer.
I am... -
Space/microgravity simulation: Extract DNA for MinION
Hello,
I hope to collect samples from lava tubes (microbial mats on the walls and nearby soils) to perform a metagenomics analysis. I will... -
AMPure XP clumps during Cas9 library prep
Hi everyone,
I'm using the ONT CRISPR-Cas9 targeted enrichment protocol (https://www.nature.com/articles/s41587-020-0407-5) to sequence a 18kb... -
Estimation of genome size from Nanopore using jellyfish
Hi,
I would like to calculate the genome size from the whole genome ONT nanopore reads (~8.7 GB) for a plant genome. My aim is to assemble the... -
SPADES: Failed to determine erroneous kmer threshold. Threshold set to: 22
Hi,
Thank you for developing SPAdes for hybrid assembly.
I tried to assemble Illumina pair-end with Oxford Nanopore Sequence for... -
Long-read Nanopore Sequencing course
GENOME ASSEMBLY USING OXFORD NANOPORE SEQUENCING
Free University (FU) Berlin - 10 – 14 February 2020
This course will... -
Discovering the world of the Long-read nanopore Sequencing
Nanopore sequencing technology is an emerging and a promising sequencing technology, which promises high sequencing throughput, low cost and longer read... -
Assembling haplotypes of a highly heterozygous gene cluster with canu
I am trying to assemble haplotypes for a peculiar region of the human genome that (1) has high heterozygosity, (2) has variation in presence or absence... -
Genome assembly using oxford nanopore sequencing
Course: GENOME ASSEMBLY USING OXFORD NANOPORE SEQUENCING
Course website: https://www.physalia-course... -
Hannover, Gemany. PhD position in translational implementation of metagenomics
PhD position in translational clinical implementation of metagenomics
A PhD position is available for 3 years in the Research Core Unit... -
Demultiplex nanopore reads with custom barcodes
We have a problem trying to demultiplex MinION sequences with custom barcodes. Do you have any software recommendations we can try for demultiplexing...
