Hi there,
We consistently conduct multiplexed shotgun metagenomic sequencing (human stool samples) using an Illumina PCR-free protocol....
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16s v4 region library preparation with negative control
Hi.
I am about to run 16s v4 region for bacterial population comparison between groups.
I used Illumina nextera index and kit earlier....Last edited by trong.wised; 03-08-2017, 08:39 AM.
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pyrosequencing decreasing/sloping curve in negative control
Is anyone having similar problem and could give me answer why sometimes curve in negative controle is decreasing and sometimes is straight while checking...