Hi all
As our NextSeq500 is old, We could replace it that we used by a NextSeq 2000.
I would like to know your opinion...
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what is your opinion about the NextSeq2000?
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NaOH denaturation with large volumen of low conc library
I am doing targeted sequencing using a <15000 kb panel, and because I consequently get rather low library conc (and try to limit the no of PCR cycles)...Last edited by madras; 09-13-2018, 09:09 AM.
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NextSeq - Banded Clustering images
In September, I noticed that our NextSeq is producing a very odd banded clustering image. Specifically a there's a very bright band of clusters through...
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Making a custom run recipe on nextseq 500
Is it possible to design a complete custom run recipe by yourself on a Nextseq500. For instance I am considering a PE sequence run were some specific...Last edited by provirus; 10-17-2017, 10:04 PM.
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bcl2fastq error "No tiles were found to process"
Hi,
I am quite a newbie for bcl2fastq software. I obtained my data from NextSeq500 system and trying to run bcl2fastq 2.0 for fastq conversion....
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Low number of variants taken from high GB data- NextSeq 500
Hi,
I got 112GB raw data from nextera rapid capture enrichment of nextseq500. FastQ files are approximately 7-9 GB. When I try to annotate...Last edited by GizemGoles; 06-19-2017, 11:31 PM.
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Nextseq500 N's from position 25
Hi,
I've been seeing a lot of Nextseq500 runs lately and I found a very disturbing phenomenon. In a lot of the runs I start seeing a lot of N's...