I have received a project and could use some advice.
My task is to find sequence matches in mRNA databases across 20+ taxa. I was presented...
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Finding transcriptome matches for thousands of 21-mers
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High percentage of Off-target reads
Hello,
we are using TruSight Capture Kits from Illumina for Targeted Sequencing.
Normally we have around 25% off-target reads within... -
Finding CRISPR gRNA off-target matches using NGS mappers
Hi all,
To keep it brief I have about 3 million, 23bp sequences (gRNAs) which are putative CRIPSR-Cas9 sites, all of which I need to screened...
