The...
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Problem with ONT sequencing pipeline. Probably in the Samtools part
Hi everyone. I have two sets of .fastq files from two ONT sequencing runs and one of them isn't working after generating .bam files.
The... -
Alignment of ONT data in a Java program through processBuilder
I have a Java-based program for alignment and variant calling. So far we have been using only illumina reads and relied on Bowtie2, which generated great... -
somatic SNV tool for ONT samples
Hello,
I'm looking for a tool to call somatic single nucleotide variations on reads from an ONT run.
I have both tumor and normal samples... -
Discrepancy between Qbit quant & read count - possible contamination?
We are performing MinION sequencing of DNA extracted from fresh water samples.
We got some preliminary results that suggested possible contamination,... -
split concatenated reads from different samples
Hi,
I have ONT data (fastq, 1 run) that consists of the same long range amplicons for several samples. PCR primers are identical for all... -
Nanopolish 1D
I am working on 1D minion data, albacore 2.0 called, and attempting to use nanopolish on canu derived contigs.
I am trying to follow the github/readme... -
New ONT System: GridION X5
https://nanoporetech.com/products/gridion
ONT has two separate purchase models: CapEx ($125k upfront + $300 per flow cell) and OpEx ($0 upfront...Last edited by gringer; 03-15-2017, 01:51 AM.
