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display orientation of sequences in alignment
How can I display the orientation of sequences in a blast alignment? In particular, I am using Bl2seq to align multiple sequencing results and primers,... -
Illumina MiSeq read orientiation
Hi all,
I've got RNA-seq paired-end Illumina MiSeq data. I'm stuck with which read now represents the forward strand, and which one the reverse...Last edited by Puhekupla; 02-26-2014, 04:03 AM. -
Paired end RNA-seq orientation
Can someone explain to me what the orientation in paired-end RNA-seq data means? Is forward/forward the standard orientation? How does this affect tophat... -
Tophat library type and pair orientation for illumina data
I have some questions about read orientation in Tophat for Illumina data that I cannot find the clear answers yet and I hope you can shed some light on... -
Reads Orientation
Hello,
Can someone briefly tell me what orientation can have pair end reads?
Can the have all of the following orientation?
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Direction/Orientation of Illumina read in SAM file
Documenting my 3 hours spent trying to understand what parameter in SAM file tells read orientation in the hope it will turn out to be helpful to someone.... -
paired-end question
Hi,
I am trying to figure out Solexa paired end read output. Can someone please tell me if my toy example is correct?
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