Dear All,
I am pretty new for Illumina Miseq platform.
The results I got are demultiplexed paired end reads. It has been quality filtered...
3 results in 0.0020 seconds.
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Illumina MiSeq, too many OTUs
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Qiime parallel_pick_otus_uclust_ref.py
Good morning everyone!
I ran parallel_pick_otus_uclust_ref.py with a high quality fasta file and silva.nr.v119.align as the refence file.... -
Bacterial species definition / OTUs
So, generally people apply 97% similarity cut-off for species/OTUs in 16S based metagenomics. As far as I know, the 97% cut-off became standard practice...
