Hello,
I'm sequencing 16S libraries (prepared and sequenced according to the EMP protocol) on MiSeq using a V2 kit. We used to load 12pM...
3 results in 0.0021 seconds.
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Non-responsive over clustering issue
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Terrible overclustering -- 16S EMP protocol
Hi SEQ-users,
We've been using the following protocol for 16S V4 region amplicon sequencing on MiSeq, which I think is very familiar to... -
Overclustered sequencing (low PF, low Q30) V2 2x250
Over the weekend I set up a V2 2x250 16S sequencing run on our Illumina MiSeq (I work in a Core lab). I came back on Monday and the results were terrible....
