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absence of overlapping nucleotides
Hello NGS community. I have a question that I'd greatly appreciate your ideas on. We recently conducted an RNAseq analysis for a samples with a specific... -
merge or do not merge overlapping paired-end reads?
Dear all,
I would like to discuss with you, if it is truly meaningful to merge overlapping paired end (PE) reads from Illumina exome or whole...Last edited by evakoe; 07-20-2015, 03:05 AM. Reason: additional argument, added link to another thread -
How can a short read be assigned to a particular transcript isoform?
Okay, first post, I hope I can explain this. I am interested in transcripts that encode ERCC5 isoforms. and according to this (and other sources), there... -
overlapping in PE library sequencing
Hello,
I'd like to do de novo assembling of a fungal genome(~20 Mbp).
I will use Miseq data for the assembling.
I found... -
lots of overlapping reads - Pair-end library from Nextera
We have done a couple of run (2X150 and 2X250) of Miseq on bacteria genome sequencing. They all have a high overlapping rate (>80%).
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