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Targeted sequencing workflow?
I have a non-barcoded multiple samples pool of long amplicons sequenced on RSII. I'm trying to figure out the correct workflow. My goal is to perform...
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Pilon ambiguous base handling?
Hi folks,
I'm using pilon to polishing a pacbio assembled genome with illumina data, and I'm confusing about how pilon treats single "N"...
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Falcon PacBio assembler error
Hello,
I am trying to assemble a genome using Falcon's Pacbio Assembler. I keep running into an error and 1 job is left incomplete. I am...
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Help: de novo assembly using SMRT portal and hgap.3
Problem: Extremely new to assembly and attempting to execute de novo assembly using hgap.3 in SMRT portal using 9 SMRTcells. lambda example data provided...
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Correcting PacBio with a hybrid approximation
Hello everyone,
I have PacBio data and I would like to correct them with Illumina HiSeq short reads. I test several programs and finally...