Hi all,
I have a question on calculating the overall depth of coverage for exome or targeted panel sequencing samples.
As a simple...
7 results in 0.0021 seconds.
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Calculating depth of coverage for overlapping paired-reads
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how can I retrieve one read sequence?
Hi, I use Integrative genomic Viewer to find one Read which mate is mapped=no.
This no mapped pair-end read may be a result from structural variation.... -
how to verify large number corrected reads is correct?
Hi all,
I wrote a program of correction to correct pair-end reads.
But the method of verifying corrected reads is difficult for... -
Segmentation fault (core dumped) in Velvet
Hi all,
I am using MiSeq paired end data which I recived from our sequencing facility, this data had already been cleaned using sythe and... -
Weird PNEXT and RNEXT values in Bam from BWA
Hi All,
I'm using BWA to align some paired end illunmina data. The reads vary in length, but around 45-50bp. I fully expect that a large... -
Merging paired end reads (R1 and R2 files)
To anyone who may have dealt with Illumina MiSeq paired end reads: what are the best programs/scripts to use when merging the R1 and R2 (forward and reverse)... -
whether should keep same number of mate reads as input into Tophat
Hello all,
I use fastx-toolkit to fliter low quality reads from paired-end reads files respectively, which will lead to the different amount...
