Hi, I'm new to genomic data and I'm just learning the basics of Illumina HiSeq and the resulting data. There's one thing I'm confused about.
...
48 results in 0.0024 seconds.
-
basic Q about paired end reads from HiSeq
-
Problem: "Pair-end" reads scRNA seq data (Drop-seq)
In case of Drop-seq, we have paired end data.
Read 1: Cell code + UMI (unique molecule identifier)
Read 2: The transcript... -
low fluorescence intensities read 2, HiSeq 2000
hi everyone
I have a problem with PE module Hiseq 2000
Basically the instrument read well the read 1 and the read index, but when arrive... -
MiSeq Paired End Reads for Draft Assembly
Hi Everyone,
I am trying to de novo assemble a draft bacterial genome with a single chromosome from another persons data. The insert size of the...Last edited by cktheory; 12-23-2015, 08:06 PM. -
inline barcodes appearing in reverse read of pair
Hi All,
I have Illumina 101bp paired-end data where the libraries were prepared with custom inline barcodes at the P5 end. So the first... -
overlapping paired-end reads vs single end reads
Dear all,
I am doing 2x100 paired-end (PE) exome sequencing on an Illumina machine with the Nextera rapid capture exome capture protocol.... -
BWA alignment using paired end reads from different lanes
Hi,
I would need some help on to proceed for WES analysis.
I observed from different posts in the forum that the paired... -
Convert paired-end, longer reads to single-end, shorter reads
Hi,
Possibly a very dumb question, but if I have a dataset of paired-end, 150bp reads, but a computational tool that is formatted to analyze single-end... -
Draft genome scaffolding with RNAseq paired-end reads
Hello all,
I used Tophat to map 100bp, PE Illumina transcriptome reads to a draft genome (133062 contigs).
Our main goal was SNP... -
Sample FASTA data and questions about paired end reads
Hello,
I have some basic questions I think.
1. Where can I find some sample FASTA files? I mean something like a file... -
Extracting insert size from .bam using pysam
Hi there, this is my first time posting. I am fairly new to bioinformatics so I may make silly errors here and there.
Anyway, I have been... -
bwa mem - paired reads
Hi,
I'm still a fair NGS newbie & I'm trying to run bwa mem (v0.7.9a) on some 91bp paired end sequences & get the following encouraging... -
'Hallo' from Germany!
Hi all!
I'm a PhD-student in Germany and work with small eukaryotes that thrive within ocean currents (Plankton). In one of my projects... -
Filtration of plasmid metagenome paired end reads
Hi all,
I am studying plasmids metagenome from clinical samples. The plasmids were captured from metagenomic DNA by digestion of linear... -
How to deal with paired end reads with one mapped, the other not
Hi all,
I am using gsnap to map paired end reads to reference, and got sam file as output. However, it seems that in the file, there are some pairs...
