Hello!
can someone please give an opinion: does that make sense to merge PE reads before a de novo RNA-seq assembly (say with Trinity)?
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Merging PE reads before de novo RNA-Seq assembly
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How to "chop" a large FASTQ PE file in half?
Hello,
I hesitate to use the following terms: "split", "partition", "trim" - because they all have special...
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Illumina_PE_reads-how to add the barcode sequence of read1 to the beginning of read2
I have read1.fastq and read2.fastq from the same lane.
At the beginning of read1, there are 7-9bp barcodes, but no barcode in read2.
so...
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Tophat can't find read-pairs??
Hi, got a question that may be a very quick answer... I am using PE Illumina reads for Tophat runs, and always get output like this:
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