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Assembling haplotypes of a highly heterozygous gene cluster with canu
I am trying to assemble haplotypes for a peculiar region of the human genome that (1) has high heterozygosity, (2) has variation in presence or absence... -
Reason for max read length?
What exactly is it that limits the read length anyways?
Also, eventually some oligos get "out of phase" and then muddy the signal.... -
Samtools is failing to phase bam files accurately
My colleagues and I are assembling transcriptomes from RNASeq data. Our pipeline can (loosely) be outlined as follows:
1. De novo assembly using... -
Phase genotypes in Galaxy
I used Galaxy to generate vcf files, but the output has unphased genotypes. Are there any options for obtaining the phased genotypes through Galaxy?...
