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African_Skies started a topic MiSeq still underclustered at 18 pM

in Illumina (Solexa)

12-10-2018, 09:31 PM
MiSeq still underclustered at 18 pM

Hello dear people,
We are doing some whole genome sequencing on a plant genome using the MiSeq with v3 reagents for 2x250 paired-end. Library size...

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Justin87 started a topic 16S Sequencing Run - Under-Clustered; Higher PhiX Alignment than expected

in Illumina (Solexa)

02-12-2018, 02:39 AM
16S Sequencing Run - Under-Clustered; Higher PhiX Alignment than expected

Good day,

We recently performed a 16S metagenomics sequencing run (288 samples) on the Illumina MiSeq using a v3 (600 cycle) kit.
...

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