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What does PHRED stand for?
I know it seems like a silly question to ask, but Google has no answer for me, nor do the papers published by Phil Green and Brent Ewing themselves. The...
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bowtie2 Saw ASCII character 7 but expected 33-based Phred qua
I'm running bowtie2 on a windows 7, 64 bit machine. My reads are prokaryotic paired end reads derived from an Illumina HiSeq instrument
below...Last edited by swatve3; 02-16-2014, 12:35 PM.
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Hello from a Sanger sequencer
Hi,
My name is Ann, and I'm an old Sanger sequencer getting used to the NGS world. I used to do radioactive (S-35 and P-33) sequencing, ~3-5 days...
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Quality filtering
I am new to the field but from what I gathered is that it is essential to filter sequences with low quality (phred scores).
But what I...
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hello and first questions!
Hello there!
I'm starting with my brand new data from Roche's 454 pyrosequencing and I have some questions at this point.
...