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What does PHRED stand for?
I know it seems like a silly question to ask, but Google has no answer for me, nor do the papers published by Phil Green and Brent Ewing themselves. The...
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Hello from a Sanger sequencer
Hi,
My name is Ann, and I'm an old Sanger sequencer getting used to the NGS world. I used to do radioactive (S-35 and P-33) sequencing, ~3-5 days...
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bioinformatics
Hey guys!
I was wondering how should I interpret the quality scores coming from results from 454 GS jr cDNA (not paired end) reads?...
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Phred score of i
Hi
I have the following sequence off of a paired end HiSeq2000 run:
@UNC11-SN627_0113:1:1101:1382:2135#0/1
TTAAGGTTTTCACTGGTATAAATCTTTCTATATTGAGACAAAATTTAAGA...