Hi all,
When I was using the RSEM to align my reads.fastq to my transcripts.fasta (trinityrnaseq_r2013-02-25/util/RSEM_util/run_RSEM_align_n_estimate.pl),...
3 results in 0.0046 seconds.
-
Help with RSEM: how to change default phred33 to phred64
-
Bowtie2 SAM output missing base quality scores
I've been using the latest version of Bowtie2 to map 100 base Illumina reads for RNA-seq. I am also trimming 25 bases from the 3' end.
... -
Detecting FASTQ Quality Score Format
Hello,
I'm trying to determine whether FASTQ reads quality scores are encoded as phred64 or phred33. The naive method I came up with was...
