Hi all,
i am wondering what are possible causes of low Q30 scores, apart from overclustering?
I am working with amplicon...
7 results in 0.0029 seconds.
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quality score issues
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Tandem dual index- low Q30 for index 2
Hello,
While reading up about index hopping, I came across the Illumina whitepaper (https://www.illumina.com/content/dam/illumina-mark... -
iSeq, High Q30% but Low PF%
So there's not a lot of data for iSeq since its pretty new - but if anyone has had this issue on MiSeq or in general sequencing please help!
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AmitChaurasia started a topic I had a Metagenomic 16s rRNA run on MiSeq v3 kit 2 x300 cycles. with good PF >89% butin MetagenomicsI had a Metagenomic 16s rRNA run on MiSeq v3 kit 2 x300 cycles. with good PF >89% but
Hi All,
I had a metagenomic run on MiSeq using v3 kits of 16s rRNA v3-v4 region. I have loaded 8pM pooled library with 10% PhiX spike-in.... -
HiSeq 2500 v4 chemistry Read 1 quality drop
We have been seeing a very specific fail mode in about 1 in 10 runs since upgrading to v4 chemistry last year, and we wonder if anyone else is experiencing... -
MiSeq run with low Q30 and high cluster PF
Hi all,
Could anybody give me an idea or though on the following?
I made a run with the MiSeq using the TrueSeq LT kit and... -
Ion Proton Q20
Hello All, we've only just done our first Proton run and the per base quality of the FastQC has ~90% of our data in the Q20 to Q28 range, should I be...
