Hi all,
I have recently prepared a pool of 9 ATAC-seq libraries from mouse hearts and performed a MiSeq Nano run to validate my pooling...
10 results in 0.0031 seconds.
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QC of ATAC-seq libraries on MiSeq Nano
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QC of ATAC-seq libraries on MiSeq Nano
Hi all,
I have prepared a pool of 9 mouse ATAC-seq libraries for HiSeq sequencing, but our facility manager strongly recommended running... -
bcl2fastq2 html output column meanings
Hi,
I have a question concerning the bcl2fastq2 html output. I'm attaching an example lane summary table found in the html output.
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Determining quality of ChIP-seq data
Hi all,
I am brand new to ChIP-seq, and I'm doing some practice data analysis. I'm trying to determine whether any trimming or post processing... -
Fastx and Soapdenovo trans
Hello,
I have filtered my illumina pair-end reads (Forward lib-24 million reads, Reverse Lib 24 million) using FASTX_Quality_Filter by... -
FASTQ read filtering/trimming/subsets ... a new approach
Please try MEEPTOOLS and post your comments on how we can improve it.
Thanks.
https://github.com/nisheth/meeptools... -
ChIPQC: a package for assessing quality of ChIP-seq samples and experiments
In conjunction with my colleague, Thomas Carroll, who has been looking at quality metrics for ChIP-seq libraries on a large scale (including evaluating... -
RNASeq: Read length different from expected
Hello all,
I have received paired-end reads for 40 samples.
The reads are supposed to be 100bp per end.
Instead, 20 of my... -
Is my chip-seq data garbage?
I received some chip-seq data that had a very high amount of sequence duplication (over 90% of the reads). The experiment was looking at H3K4me3. I aligned... -
Platform independent quality assessment of Exomes
Hi,
we are working on a platform independent score to assess the quality of an exome. We predict the genotyping accuracy of the exome simply based...
